Abstract by Cody Roberts
Chemistry and Biochemistry
The Role of CMG2 Cytosolic Tail in Mediating Endocytosis
Capillary morphogenesis gene 2 (CMG2/ANTRX2) has been identified as a potential therapeutic target to inhibit pathological vessel growth that is important in cancer and other diseases, however the mechanism of action is not fully understood. It is believed that one potential function of CMG2 in angiogenesis is endocytosis of matrix fragments. To further investigate this function, we identified key intracellular regions of CMG2 required for endocytosis using anthrax protective antigen (PA) as a model CMG2 ligand. Various mutants and truncations of a C-terminal mClover fusion of CMG2 were created for further study. Assays were done with the transfected cells by treating with PA-Cy5. Results indicate that the cytosolic tail has a role in mediating endocytosis. The current model suggests that CMG2 requires ubiquitination of K350 for clathrin mediated endocytosis. Contrary to the ligature, we observe that CMG2 mediated endocytosis does not depend on ubiquitination of K350.