Abstract by Elaine Lazalde
Chemistry and Biochemistry
Detecting Sepsis Related DNA Using Fluorescence
Drug resistant sepsis is a growing problem worldwide as 23,000 people in the US die each year according to the CDC. Patients with sepsis must receive treatment quickly; however current tests can take 48 hours. We are creating a diagnostic system for determining bacterial drug resistance that will reduce this time. A key part of this system is to capture DNA sequences related to drug resistance. After capture, the DNA will be fluorescently labeled and quantified in a fluorescent detection system. We capture the DNA by using magnetic beads modified with single stranded DNA sequences that hybridize to target DNA. According to recent results, targets strand can be most effectively captured when beads are modified with a density of 44 capture strands per bead. In order for the process to be successful we need to have an overall efficiency of 81%. Preliminary data shows an efficiency of 53%.