Abstract by Tsz Yin Chan

Personal Infomation

Presenter's Name

Tsz Yin Chan

Degree Level



Chrissy Egbert
Logan Larsen
Kristina Kohler
Will Tenney

Abstract Infomation


Chemistry and Biochemistry

Faculty Advisor

Joshua Andersen


A novel 14-3-3ΞΆ-dependent mechanism of TNK1 regulation that promote cancer tumorigenesis


The oncogenic phospho-binding protein 14-3-3ζ acts as a scaffolding protein to promote cell growth, survival, and motility. We recently discovered an interaction between 14-3-3ζ and the non-receptor tyrosine kinase TNK1. Although TNK1 has been identified as an oncogenic kinase in multiple genome-wide screens, little is known about its regulation and function. In our study, we identify a cluster of three phosphorylations on TNK1 that promote 14-3-3ζ binding and hence boost TNK1 kinase activity to promote migration of cancer cells. In addition, we discover that the removal of TNK1 C-terminal UBA domain renders TNK1 as a highly active kinase. Finally, we identify mutations in primary human tumors that produce a truncated TNK1 that lacks the UBA domain but possesses constitutive kinase activity. Our current work is focused on understanding the contribution of these mutations to tumorigenesis.