Abstract by Matthew Teynor
Chemistry and Biochemistry
Pam Van Ry
Galectin-1 protein therapy increases the membrane repair capacity in cell models of LGMD2B
Galectin-1 (Gal-1) is a small, multifaceted protein found naturally in oxidized and reduced forms, and as a monomer, dimer and multimer. Each of these forms have unique biochemical functions, and of particular importance is the carbohydrate recognition domain (CRD) present in the reduced form. Our lab currently focuses on assessing the therapeutic potential of the different forms of Gal-1 in limb-girdle muscular dystrophy type 2B (LGMD2B). LGMD2B is a muscle wasting and weakening disease caused by mutations to the dysferlin protein. It is known that the clinical symptoms of LGMD2B are directly caused by decreased muscle membrane repair due to lack of functional dysferlin. Through a laser ablation assay we show that the CRD of Gal-1 participates in increasing membrane repair capacity in cell models of LGMD2B, thus providing evidence for the therapeutic value of Gal-1 in LGMD2B.