Abstract by Dhananjay Patel
Chemistry and Biochemistry
Fabrication of Novel, High Capacity, Coated SPME Fibers by Sputtering and Thin Film Deposition.
Solid phase microextraction (SPME) is a solvent-free sample preparation technique used in GC (Gas Chromatography) and HPLC (High-Performance Liquid Chromatography). SPME fibers can be coated with various solid and liquid extraction phases. Recently, we have used sputtering of silicon to deposit novel and highly porous SPME coatings. This presentation will be focused on an extensive study of the relationship between the porosity and thicknesses of the silicon coating and the extraction efficiency of fibers. It will also discuss new stationary phase coatings (e.g., thin polymeric coatings). These new fibers/coatings and/or model/witness substrates that represent them have been characterized by spectroscopic ellipsometry, water contact angle goniometry, and X-ray photoelectron spectroscopy. The performance of the resulting SPME fibers in both headspace and direct immersion modes is demonstrated. Various specific applications are discussed, e.g., analysis of PAHs. These fibers showed better stability, higher capacity, more reproducibility, better robustness, and no carryover in comparison to the commercially available fibers.