Submitted Abstracts
Abstract by Richard Carson
Personal Infomation
Presenter's Name
Richard Carson
Co-Presenters
None
Degree Level
Doctorate
Co-Authors
None
Abstract Infomation
Department
Chemistry and Biochemistry
Faculty Advisor
John Price
Title
A Multi-omics Analysis of Lifespan Extension in Mice Through Short-term Calorie Restriction Reveals Mechanisms of Post-Transcriptional Proteome Regulation
Abstract
Calorie restriction is one of the best-studied methods for extending longevity in laboratory animals, but the mechanism remains incompletely understood. Our lab combined RNA-Seq, RNA kinetics, quantitative proteomics, and kinetic proteomics in a novel unbiased application to calorie-restricted mouse tissue samples. An overall slowing of protein synthesis was observed, with evidence suggesting that alterations in tRNA metabolism are responsible: aminoacyl tRNA synthetase metabolism was found to differ significantly between calorie restriction and ad libitum diets, indicating changes in the available tRNA pool for protein synthesis. We hypothesize that decreasing the pool of charged tRNA available for translation allows the cell to lower both its energy consumption and protein synthetic burden, leading to the improvements in cellular and organismal health observed under calorie restriction.