Abstract by Connor Holman

Personal Infomation

Presenter's Name

Connor Holman



Degree Level



Max Jones
Monique Spiers
John Price

Abstract Infomation


Chemistry and Biochemistry

Faculty Advisor

John Price


Variations in sphingolipid rheostat and pathway between cancerous and non-cancerous pancreatic cells


Pancreatic cells alter their use and production of bioactive sphingolipids. The balance between C16 ceramide (cer) and sphingosine-1-phosphate (S1P) is quintessential in maintaining normal apoptotic and proliferative function of pancreatic cells. Obstructing this balance can lead to rampant proliferation; ergo cancer. Culturing pancreatic cells, both cancerous and immortalized human cells, and measuring their sphingolipid rheostat, or more specifically the balance between cer and S1P, results in a more complete understanding of the nature of numerous strains of cancer. This quantification can be measured accurately using tandem liquid chromatography- mass spectrometry techniques. Furthermore, using isotopic labeling, we can discover the pathway of sphingolipids and how they differ between human cells and cancer cells and gain a better understanding of differing cell pathways between cell types, both cancerous and non-cancerous alike. Quantifying this sensitive sphingolipid balance using LC-MS techniques and following the sphingolipid metabolic pathway using isotopic labeling will grant significant progress in lipidomic cancer research.