Alzhiemers disease is currently difficult to detect using biochemical methods, especially in its early stages. Our research used a serum peptidomic approach to examine low level, low MW molecules. To remove high abundance serum proteins, acetonitrile precipitation was used. Each sample was analyzed by cLC-ESI-QTOF-MS/MS using reverse phase liquid chromatography. We used a Students t-test to compare cases and controls initially.  In the first study 59 markers were found, 13 being particularly good.  In a second study these 13 promising markers were analyzed and 4 showed marked differences in early stage AD. Of interest there appears to be gender differences among the 13 AD markers. New specimens will be analyzed to confirm and expand gender-specific biomarkers to diagnose individuals with early stage AD.  This ability is critical for meaningful drug trials.