Abstract by Cody Roberts
Chemistry and Biochemistry
Identifying CMG2 Intracellular Interactomes
Capillary morphogenesis gene 2 (CMG2/ANTRX2) has been identified as a potential therapeutic target to inhibit pathological vessel growth that is important in cancer and other diseases. While our lab has shown that CMG2 is an important regulator of angiogenesis, the intracellular regulatory pathway has not been identified. To begin to establish a mechanism for the antiangiogenic effects observed when normal CMG2 function is blocked, we have performed a BioID or proximity proteomics experiment to identify potential CMG2 interactors. Determination of the CMG2 interactome will help elucidate both the physiological role CMG2 and the pathway whereby it is a central regulator of angiogenesis. To do this, we have treated human cells expressing a C-terminal CMG2 biotin ligase (BirA*) fusion and treated cells with a mutant of anthrax protective antigen that is known to inhibit angiogenesis in vitro and in vivo. We will discuss the proteins identified in these experiments and present our current model describing CMG2’s role in angiogenesis.