BYU

Abstract by Karyna Howell

Personal Infomation


Presenter's Name

Karyna Howell

Degree Level

Undergraduate

Co-Authors

Haifa Almughamsi
Daniel Ballif

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Adam Woolley

Title

Immunoaffinity Extraction of Thrombin Anti-Thrombin in 3D printed Microfluidic Devices

Abstract

Preterm birth (PTB), birth before 37 weeks of pregnancy, affects 15 million births worldwide annually; yet there is no rapid diagnosis method. These premature babies miss vital development during the final weeks of pregnancy, leaving them with lifelong health complications. Our collaborators identified a panel of nine PTB biomarkers. We are developing 3D-printed microfluidics devices that rapidly isolate, label, and detect these biomarkers. This research focuses on the immunoaffinity extraction of the PTB biomarker thrombin anti-thrombin (TAT). We identified an antibody with a high affinity for TAT via dot blot. We then form monoliths in 3D printed devices using UV polymerization, and covalently immobilize TAT antibody. We are evaluating TAT capture and elution in 3D printed microfluidic devices in order to determine a woman’s risk for PTB.