BYU

Abstract by Ashley Markham

Personal Infomation


Presenter's Name

Ashley Markham

Degree Level

Doctorate

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Pam Van Ry

Title

Proteomic Analysis for Treatment of Limb-Girdle Muscular Type 2B Model with Galectin-1

Abstract

Limb-girdle muscular dystrophy type 2B (LGMD2B) is dysferlinopathic muscular dystrophy which affects 1:1,300 to 1:200,000 people every year in the muscles of the limbs and girdles, beginning in adolescence with chronic progression. Dysferlin seems to be involved with membrane repair, but how mutations to the dysferlin gene (DYSF) result in LGMD2B is unknown. The purpose of this research is to identify changes in the proteome of dysferlin deficient cells with an upcoming therapeutic, galectin-1, to increase membrane repair in muscular dystrophy models. We used proteomic analysis to identify proteins whose regulation and turnover rate was different through treatment with galectin-1. We found upregulated proteins in areas of cellular migration and cellular differentiation. Our research provides an insight into how galectin-1 is modifying the capacity and function of dysferlinopathic muscle cells.