Abstract by Samuel Squires

Personal Infomation

Presenter's Name

Samuel Squires

Degree Level



Peyton Bishop

Abstract Infomation


Chemistry and Biochemistry

Faculty Advisor

Ryan Kelly


Optimization of Sample Preparation Technique for Single Cell Proteomics


Mass spectrometry based proteomics has become a powerful approach for resolving cellular heterogeneity, which is crucial for understanding cellular and disease mechanisms. The recently developed Nanodroplet Processing in One pot for Trace Samples (nanoPOTS) workflow enables high-resolution proteome coverage of small-cell populations. Despite its application in a number of nanoscale proteomic studies, however, the fundamentals of the technique have remained unchanged for single-cell sample processing. Here, we aim to optimize sample processing to achieve a depth of coverage of >3000 proteins/cell for single-cell studies. By manipulating sample volume, nanowell surface area, and reagent concentrations, we will achieve shorter processing times and increased sample recovery, enabling high-throughput proteomic analysis of single cells with far greater efficiency. As a result, higher resolution of cellular heterogeneity can be achieved in less time, providing an enhanced tool for studying, diagnosing, and guiding treatments for disease.