BYU

Abstract by Thy Truong

Personal Infomation


Presenter's Name

Thy Truong

Degree Level

Doctorate

Co-Authors

Enoch Councill
Yiran Liang
Yongzheng Cong

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Ryan Kelly

Title

High throughput NanoPOTS

Abstract

For nanoscale proteomics, one of the biggest challenges is to reduce sample-processing volume while maintaining the recovery rate. NanoPOTS platform was able to achieve that goal by downscaling processing volume to < 200 nL to minimize surface losses. When couple with LC-MS, it enables identification of ~1500 to ~3000 proteins from ~10 to ~140 cells, respectively. However, the remaining obstacle is the low throughput, which is mainly due to manual samples loading step. Therefore, coupling the nanoPOTS with a commercial auto sampler would greatly enhance the efficiency. In this work, a 10-port valve and an on-line SPE were employed for automation. For interfacing between the auto sampler and the nanoChip, we fabricated a 3D-printed plate instead of a regular 384-well one. This platform would maximize the numbers of analysis per day without manual loading, as well as reducing the sample-loading time into the SPE since it can be simultaneously performed while the analytical column undergoes regeneration. Moreover, it eases the need to assemble/dissemble the SPE and column after each analysis; thus; reducing incidents of SPE and column wearing out /clogging.