BYU

Abstract by Parag Gajjar

Personal Infomation


Presenter's Name

Parag Gajjar

Degree Level

Doctorate

Co-Authors

Shi Liang
Curtis Jeffrey
Benjamin Smith

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

James Moody

Title

Structural characterization of radical SAM enzyme complexes

Abstract

Radical SAM enzymes use an iron sulfur cluster and SAM to generate a radical that enables difficult reactions. Ribonucleotide reductase activating enzyme (RNR-AE) is responsible for the activation of ribonucleotide reductase. We are working on finding the structure of RNR-AE and RNR bound to RNR-AE by X-ray crystallography and cryo-EM. This will give structural information like the interaction site of both enzymes and the bound position of the iron sulfur cluster in RNR-AE. We are also working on another radical SAM enzyme complex involving hydrogenase maturases. Hydrogenase uses a special H-cluster to form or reduce hydrogen and surprisingly this cluster is formed by three different maturase enzymes called Hyd E, F and G. We don’t have much information about how they interact and form the H-cluster during the maturation process. We are using cryo-EM and Mass spectrometry to find the structure and interactions between the Hydrogenase maturases respectively and these data may be help full to solve maturation mystery.