Abstract by Jacob Gill
Chemistry and Biochemistry
Single-Cell Lipidomics in Liquid Chromatography Mass Spectrometry
Lipidomics provides information on the lipids involved in cellular structure, energy storage and signaling. Because lipid compositions are abnormal in diseased cells, single-cell lipidomics will allow for the determination of disease and will advance medicine. Liquid chromatography (LC) combined with mass spectrometry (MS) has been used by others to analyze large mammalian cells with limited success. We aim to accomplish accurate and precise detection of lipids in single, average-sized mammalian cells through LC-MS. However, to sufficiently increase our limit of detection, current cell preparation protocol must be refined to minimize the loss of lipids due to surface contact. Here we show that our current method identifies lipids from 1,000 cells. Our future methodology will give in-depth and accurate information of the lipidome of a single cell, thereby furthering progress in other fields of science. Eventually, single-cell lipidomics will create a means for less-invasive yet more accurate biopsies. Thus, single-cell lipidomics may be a means to further progress treatment of a variety of diseases.