Abstract by Jared Simmons
Chemistry and Biochemistry
Proteomic Analysis with NanoPOTS and Mass Spectrometry Imaging of Biological Tissues
NanoPOTS is a novel technology which allows the preparation of trace biological samples for proteomic analysis by LC-MS and other applications. In collaboration with the David Muddiman Lab at NCSU, we are studying differences in proteome expression across various regions of rat brain tissues. This is being done in conjunction with infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) which will be used for mass spectrometry imaging (MSI) of small molecule and metabolite levels across the tissue. Combining MSI and proteomic data collected using nanoPOTS will enable us to create a complete picture of the brain tissues. Regions of interest in tissue slices have been identified and isolated by laser capture microdissection and processed with nanoPOTS for proteomic analysis. Upon optimization of workflow, we will investigate the effect of flame retardant chemicals on rat placental tissue and regional changes in levels of small molecules, lipids, and proteins.