Abstract by Dhananjay Patel
Chemistry and Biochemistry
Fabrication of novel, high capacity, coated SPME fibers by sputtering and thin film deposition
Solid phase microextraction (SPME) is a solvent-free sample preparation technique that is regularly used in conjunction with GC (Gas Chromatography) or HPLC (High Performance Liquid Chromatography). SPME fibers can be coated with various solid and liquid extraction phases. Recently, we have used sputtering of silicon to deposit novel and highly porous SPME coatings. The resulting silicon/silica coatings can be functionalized with a C18 silane and polydimethylsiloxane (PDMS). This presentation will be focused on an extensive study of the relationship between the porosity and thicknesses of the silicon coating and the extraction efficiency of fibers. It will also discuss new stationary phase coatings, e.g., thin polymeric coatings from a phenyl hexyl silane. The performance of the resulting SPME fibers in both headspace and direct immersion modes is demonstrated. Various specific applications will also be discussed, e.g., the analysis of polyaromatic hydrocarbons and pesticides. These fibers showed better stability, higher capacity, more reproducibility, better robustness, and no carryover in comparison to commercially available fibers.