Abstract by Nicholas Chartrand
Chemistry and Biochemistry
3D Printed Microfluidics for Cell-Based Assays
The field of 3D-printed microfluidics is rapidly expanding, but currently lacks compatible resins for cell-based assays due to cytotoxicity of some resin components. Also, some resins like polyethylene glycol diacrylate do not promote cell adhesion and need to be functionalized for use with adherent cells. Our team developed a cell-compatible printing resin and approaches for functionalizing the polymer via plasma treatment and surface functionalization to promote cell adhesion. With a cell-compatible resin in hand, we have massively integrated active and passive printed components for cell-based assays. For example, we designed and validated a 3D-printed serial dilutor to achieve dilutions that span 3 orders of magnitude for dose-response testing. Similarly, we also developed a printed device that passively forms a stable chemical gradient suitable for chemotactic assays. Hence, deployment of 3D-printed microfluidics will make cell-based assays more accessible for cell research.