Abstract by Ekow Amakye
Chemistry and Biochemistry
PROTEOSTASIS CHANGE IN AGING MUSCLE
Muscle composition is responsible for support, protection and essential for effective locomotion. It is observed that there is loss of muscle mass as aging takes place. Slowing the aging rate with dietary restriction slows the loss of muscle mass. Our goal is to analyze the protein metabolism changes during different aging rates in mice. By fractionation, we separate protein components in muscle homogenate into mitochondrial and cytoplasmic fractions. We then analyze protein concentrations and turnover rates in these fractions. Here, we present the global effect of aging rate on protein turnover in mouse muscle. The result of this study will present an increased understanding of how proteostasis change affects the aging process in muscle tissue. This understanding will be integral in advancing techniques in improving muscle health and potentially reducing muscle loss due to aging.