BYU

Abstract by Brandon Chamberlain

Personal Infomation


Presenter's Name

Brandon Chamberlain

Degree Level

Undergraduate

Co-Authors

Nicholas Chartrand
Jonard Valdoz
Mawla Boaks
Jose Sanchez
Connor Knight
Nicholas Franks
Kenneth Christensen
Greg Nordin
Pam Van Ry

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Kenneth Christensen

Title

Organoid-on-a-3D-Printed-Chip: New Methods for Organoid Testing

Abstract

Organoids (i.e., self-aggregating 3D cell culture models) have facilitated increasingly robust tissue modeling in recent years. Also, 3D printing of microfluidic devices has recently become more viable for a variety of applications. We have combined these two developments by creating a 3D-printed chip to grow and analyze lung organoids. Our 3D-printed microfluidic device allows for normal organoid proliferation within an ultra-low adhesion polymer (polyethylene diacrylate; MW 250) device. A second-generation device mechanically stimulates organoids via high media flow rates to mimic capillary blood flow for the lung organoids—a vital step in the development of a novel lung organoid model. Finally, we utilized a highly integrated onboard 3D-printed serial diluter to demonstrate the potential for unprecedented levels of integration for on-chip organoid testing, which exploits the use of organoids in 3D-printed chips for testing organoid drug responses.