Abstract by Brandon Chamberlain
Pam Van Ry
Chemistry and Biochemistry
Organoid-on-a-3D-Printed-Chip: New Methods for Organoid Testing
Organoids (i.e., self-aggregating 3D cell culture models) have facilitated increasingly robust tissue modeling in recent years. Also, 3D printing of microfluidic devices has recently become more viable for a variety of applications. We have combined these two developments by creating a 3D-printed chip to grow and analyze lung organoids. Our 3D-printed microfluidic device allows for normal organoid proliferation within an ultra-low adhesion polymer (polyethylene diacrylate; MW 250) device. A second-generation device mechanically stimulates organoids via high media flow rates to mimic capillary blood flow for the lung organoids—a vital step in the development of a novel lung organoid model. Finally, we utilized a highly integrated onboard 3D-printed serial diluter to demonstrate the potential for unprecedented levels of integration for on-chip organoid testing, which exploits the use of organoids in 3D-printed chips for testing organoid drug responses.