BYU

Abstract by Ronald Zegarra

Personal Infomation


Presenter's Name

Ronald Zegarra

Degree Level

Undergraduate

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Pam Van Ry

Title

Construction of dGal-1

Abstract

Limb-girdle muscular dystrophy type 2B (LGMD2B) is a rare, muscularly debilitating illness. As an extracellular matrix (ECM) related myopathy, dysferlin related illnesses are due to a mutation in the DYSF gene. ECM-related myopathies exemplify the lack of dysferlin protein resulting in vesicle malformation, increases in inflammation and causing a decrease in cellular membrane repair. Galectin-1 being a small 14.5 kDA, non-glycosylated protein is ideal for protein therapy. In an effort to increase membrane repair, fixed dimeric Galectin-1 (Gal-gly-gly-Gal) was developed by way of computational analysis and gene sequencing. The addition of two permanently bound Glycines by way of N-terminal domain allows for permanent dimerization. Protein purification was acquired through a lactosepharose affinity column. Dimeric construct confirmation was acquired through western blots and coomassie staining.