Abstract by Seth Garfield
Chemistry and Biochemistry
Pam Van Ry
Vascularized Organotypic Lung Model
Lung disease is the third leading cause of death in the world. Many lung diseases involve cell interactions with the blood vessels. Though, current lung models do not have vascular structures which limits their effectiveness at modeling disease. This inability to model lung vasculature translates into poor prognosis among several lung diseases such as lung cancer and fibrosis. To solve this problem, we incorporated an endothelial cell line EAHy that was modified to express blue fluorescent protein derivative, Azurite. We grew organ mimics through a hybrid suspension culture and induced fetal lung-like vascularization through hypoxia (~5% O2) and supplementation of pro-vascular cytokines. Here, we report effective formation of endothelial-fibroblast networks resembling vasculature. Moreover, we observed transdifferentiation towards mature vascular components such as pericytes and smooth muscles. In conclusion, our model shows the first organotypic vascular lung model which will be necessary in studying several lung diseases.