Abstract by Daniel Poulson

Personal Infomation

Presenter's Name

Daniel Poulson

Degree Level


Abstract Infomation


Chemistry and Biochemistry

Faculty Advisor

Pam Van Ry


Isolation Techniques for Limb-Girdle Muscular Dystrophy 2B


Limb-girdle muscular dystrophy type 2B (LGMD2B) occurs as the result of non-functional dysferlin protein. The lack of dysferlin leads to compromised membrane repair, decreased muscle regeneration, and chronic inflammation. The mutation ultimately leads to the loss of ambulation by the third decade of life. Currently, no long-term treatment options for LGMD2B exist. One limitation in creating viable treatment options is the difficulty in isolating severely affected muscle groups for testing. The most affected muscle in this disease is the psoas, which is found deep in the hip girdle. This study examines different isolation techniques for the psoas and how these isolation techniques affect various assays that determine membrane strength and repair.