Abstract by Jacob Luddington
Chemistry and Biochemistry
Pam Van Ry
Murine Muscle Isolation for Laser Injury
Limb Girdle Muscular dystrophy type 2B (LGMD2B) is a currently incurable disease caused by a mutation in the gene that codes for dysferlin. When the protein dysferlin malfunctions, it leads to muscle wasting around the limb girdle area (hips and shoulders) due to decreased levels of muscle repair and myogenesis. The recombinant human protein, Galectin-1 (rHs-Gal-1), has shown promise as a novel therapeutic for the disease. In order to test whether the protein increased membrane repair, we treated diseased mice with varying levels of rHs-Gal-1. We then isolated muscle fibers from the mice and injured the muscles using a 405 nm laser in a confocal microscope. By comparign the amounts of dye that entred injured cells, we were able to show that muscles treated with galectin-1 undergo increased rates of muscle repair compared to nontreated diseased muscles. In my presentation, I will be forcusing on how the techniques used to isolate and prepare muscle fibers for laser injury impact the results of the assay.