Abstract by Yesman Akuoko
Chemistry and Biochemistry
Sequence-Specific Detection of Fluorescently labeled Bacterial DNA using a Microfluidic Chip
Sepsis, resulting from bacterial infection, is a growing concern globally with 30 million cases recorded annually. Traditional methods of identification of bacterial infection involve culturing bacteria from blood samples for at least 24 hours. In this research, nucleic acid sequences from bacteria were captured and detected in ~1 hour using a microfluidic platform. A laser ablated v-shaped channel ~0.6 mm deep for fluid flow was created in a poly (methyl methacrylate) device and sealed with a pressure sensitive adhesive. A 2.5 mm monolithic column containing a DNA capture sequence for Klebsiella pneumoniae carbapenemase (KPC) was used in capturing 10-100 pM concentrations of a fluorescently labeled KPC complementary sequence. The laser induced fluorescence measurements of the captured sequence during elution showed more than two-fold enrichment. The full analysis process was completed within 30-45 minutes.