BYU

Abstract by Kaitlynn Baker

Personal Infomation


Presenter's Name

Kaitlynn Baker

Degree Level

Undergraduate

Co-Authors

Robert Hanson

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Adam Woolley

Title

Multiplexed Solid Phase Extraction of DNA Sequences

Abstract

As antibiotic resistance becomes increasingly prevalent, we need a time-efficient method to detect antibiotic resistance genes in sepsis patients. The Woolley lab and collaborators are developing microfluidic devices which can separate specific DNA strands via solid phase extraction. To expand on prior work with single DNA sequences, I am researching multiplex capture of two different DNA sequences. Using centrifuge tubes and capture sequences on magnetic beads, we captured and recovered 67% of initial NDM and KPC antibiotic resistance gene sequences from the same sample. The fluorescently labeled DNA hybridized to the capture sequences, and then was thermally de-hybridized. The percent recovered had no significant variance from the non-multiplexed data. Knowing that multiple DNA sequences can be extracted using magnetic beads, we are now implementing this in microfluidic devices using monoliths containing multiple DNA capture sequences.