Abstract by Nathan Zuniga
Chemistry and Biochemistry
Tissue specific changes in protein metabolism associated with Alzheimer’s risk
The strongest genetic risk factor for the development of late-onset Alzheimer’s disease (AD) are polymorphisms in the lipid transport protein ApoE. Research suggests that different isoforms of APOE are linked to neurodegenerative and cardiovascular disease. For example, APOE4 has been associated with increased risk for atherosclerosis, Alzheimer's disease, and cognitive decline. However, further research is required to validate the effects of different APOE genotypes in lipid homeostasis and the careful interplay between different organs (i.e. liver and brain). We have used mass spectrometry (MS) to compare metabolic differences in APOE mouse cohorts. Our analysis of both membrane and cytosolic components have allowed us to observe how isoforms of APOE alter different components of the lipid metabolism. Using D2O tissue-labeling and state of the art LC/MS technology we compare changes in protein metabolism between APOE variants.