BYU

Abstract by Henry Gold

Personal Infomation


Presenter's Name

Henry Gold

Degree Level

Undergraduate

Co-Authors

Colten McEwan

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Joshua Andersen

Title

LRBA Deficiency and its affect on autophagy and ATG9A trafficking

Abstract

Autophagy is a cellular recycling process that leads to disease when it is changed. Disease can be cause in many different ways, many of which are unknown, due to mechanisms that have yet to be discovered. ATG9A is an autophagy regulator with few known interactors. To discover interactors, our lab used a biotin labeling technique called BioID and developed a bank of possible ATG9A interacting proteins.  One of the more interesting proteins was the vesicle trafficking regulator Lipopolysaccharide-Responsive Beige-Like Anchor Protein (LRBA).  Human mutations in LRBA cause dysfunctional autophagy leading to disease. We validate LRBA as an ATG9A interactor and measure how LRBA deletion affects autophagy.  Furthermore, we demonstrate that LRBA is an ATG9A trafficking regulator.  We hypothesize that LRBA disease is caused by dysregulated ATG9A trafficking and propose an experimental model to prove our hypothesis.