Abstract by Taylor Fish
Chemistry and Biochemistry
Development of Immunoaffinity Extraction of Lactoferrin in 3D-Printed Microfluidic Devices for Preterm Birth Diagnosis
Preterm birth (PTB), the most common complication of pregnancy, results in millions of adverse health conditions and infant deaths each year around the world and costs billions of dollars per year. Nine biomarkers from maternal blood serum are correlated with a high risk of preterm birth. A growing body of evidence shows that 3D-printed microfluidic devices can be used to perform rapid biomarker analysis, and therefore determine the potential risk of a PTB. In order to analyze PTB biomarkers, they must first be extracted from maternal blood serum. My objective is to optimize the extraction of one such biomarker, lactoferrin, through the use of immunoaffinity extraction. We have shown that lactoferrin exhibits nonspecific adsorption and requires many rinses for the columnto return to baseline levels of fluorescently labeled protein. I am working to optimize the composition of the rinsing buffer in order to significantly decrease the nonspecific adsorption of lactoferrin.