BYU

Abstract by Daniel Poulson

Personal Infomation


Presenter's Name

Daniel Poulson

Degree Level

Undergraduate

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Pam Van Ry

Title

Using Acid Reflux to Cause Idiopathic Pulmonary Fibrosis in Mice

Abstract

Idiopathic Pulmonary Fibrosis (IPF) is a fibrotic lung condition that affects 100,000 people annually in the United States. IPF is highly correlated with gastroesophageal reflux (GER), as 87% of patients diagnosed with IPF also have chronic GER. We hypothesize that chronic, subclinical levels of micro-aspirated gastric juices injure the lungs and can result in fibrosis. To test this mechanism, mice were aspirated with simulated intestinal fluid with active pepsin (SIF+P) for 7 days. Mice were run on an incline until exhaustion, correlating to the six-minute walk test of IPF patients. HR-μCT scans were conducted weekly to monitor lung volume and density. 21 days after initial treatment, the mice underwent a tracheostomy and were connected to a FlexiVent, which runs a comprehensive lung mechanics test. Data collected from these various endpoints suggests that SIF+P causes a functional phenotype typical of fibrotic patients, and as such indicates SIF+P micro-aspirations could be used as a physiological murine model for IPF.