BYU

Abstract by Mary Vallcillo

Personal Infomation


Presenter's Name

Mary Vallcillo

Co-Presenters

Hunter Chamberlain

Degree Level

Masters

Co-Authors

None

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Pam Van Ry

Title

Recombinant Galectin-1 Protein Therapy in Limb Girdle Muscular Dystrophy Type 2B

Abstract

Recombinant Galectin-1 (Gal-1) protein therapy was effective in Duchenne muscular dystrophy which has some pathophysiological end point measures overlapping with Limb Girdle Muscular Dystrophy Type 2B (LGMD2B).  Lack of dysferlin protein leads to diminished plasma membrane repair in LGMD2b. Gal-1 is maximally expressed during myoblast fusion and evidence show overexpression of Gal-1 results in increased fusion into mature muscle cells resulting in increased repair. In LGMD2B, muscle deterioration is accelerated by an overactive inflammatory response. We hypothesize that Gal-1 will improve membrane repair through immune modulation thus stabilizing muscle integrity/strength and increasing myogenesis in models of LGMD2B. Human recombinant Gal-1 (rHsGal-1) was produced and verified through western blot analysis and BCA Protein assay. We plan to treat LGMD2B muscle cells with rHsGal-1. Improved myogenesis will be evaluated to determine optimal dose of Gal-1. We will also record cytokines involved in the pro-inflammatory response.