BYU

Abstract by Steven York

Personal Infomation


Presenter's Name

Steven York

Co-Presenters

None

Degree Level

Undergraduate

Co-Authors

None

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Richard Watt

Title

Homocysteinylation of Transferrin in Relation to Oxidative Stress and Neurodegeneration

Abstract

Elevated homocysteine (Hcy) correlates with inflammation and oxidative stress in Alzheimer’s Disease (AD), Diabetes, and Kidney Disease. Free iron is the proposed cause of oxidative stress, but in healthy condition iron is chaperoned by the serum iron binding protein Transferrin (Tf). Hcy can bind to proteins and alter their function. We propose that excess Hcy binds to Tf and inhibits its ability to efficiently bind iron resulting in free serum iron that increases oxidative stress. We tested this hypothesis using biochemical iron loading assays and, mass spectrometry (MS) to assess if Hcy alters Tfs ability to bind iron. Preliminary MS analysis of Tf incubated with Hcy for 12hrs showed attachment of Hcy to Cys residues.