Abstract by Theo Stoddard-Bennett
Chemistry and Biochemistry
Gold Nanoparticles as Catalyst for Oxidation of NADH
Multiple sclerosis (MS) is an inflammatory disorder that damages the myelin sheath surrounding neurons. Oligodendrocytes (OL) surround the neurons and repair the myelin sheath but inflammation prevents myelin repair. Healthy OLs use glycolysis for energy and produce lactate as a precursor for myelin synthesis but inflammation slows glycolysis. It is hypothesized that methods to stimulate glycolysis in OLs will provide a treatment for MS. Recent work showed that gold nanoparticles (AuNPs) increase glycolysis in OLs but the mechanism is unknown. We propose that AuNPs alter the NAD + /NADH ratio and shift OLs toward glycolysis for energy. We demonstrate in vitro that AuNPs oxidize NADH to NAD + using UV/Vis methods. This catalytic property may have further important biological and medical properties for inflammation.