BYU

Abstract by Lara Grether

Personal Infomation


Presenter's Name

Lara Grether

Co-Presenters

None

Degree Level

Undergraduate

Co-Authors

None

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Richard Watt

Title

Lateral Flow Immunoassay (LFI) Diagnostics and Optimization

Abstract

Anemia of inflammation (AI) is caused through the overexpression of hepcidin. One emerging method to diagnose AI is to to measure hepcidin levels using a Lateral Flow Immunoassay (LFI). A LFI is optimized through multiple factors, including antibodies, antibody fixation, running buffers, conjugation, and the process of putting all these components together. Additional optimization of these parameters will enhance the ability to detect important disease biomarkers. Our lab is seeking to optimize all of these factors, including changes to conjugation, test line application, and materials used to decrease the cost of LFI manufacturing. I will report on methods for antibody optimization.