Abstract by Emily Cannon
Chemistry and Biochemistry
Generation of chemoresistant human colon cancer cell line
Colon cancer is the seconding leading cause of cancer related deaths in the United States. What prevents complete treatment is the development of chemoresistant cancer cells. These chemoresistant cells adapt and transform, by altering their own mechanisms and proteins, in a way that escapes apoptosis. Past research has been pioneered in this field by testing various cell cultures with different doses of drug inhibitors, much like those that are administered to patients today. We developed a line that is admininstered Oxalyplatin. After several months of observing the growth and development of the HCT-116 cells treated with oxalyplatin at several different dose concentrations, we have found them to be chemoresistant. Using "stable" chemoresistant cells, we can begin to analyze their proteomics. Doing so will allow us to understand how they are able to adapt to these threatening conditions. Comprehending their changes, will help us to understand what we can do as scientists, to eventually inhibit certain mechanisms inside the cells from adjusting, on a molecular level. Targeting specific change sites could lead to killing cancer cells in humans.