BYU

Abstract by Jacob Nielsen

Personal Infomation


Presenter's Name

Jacob Nielsen

Co-Presenters

None

Degree Level

Undergraduate

Co-Authors

None

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Adam Woolley

Title

Formation of Thrombin-Antithromin Complex for Separation of Preterm Birth Biomarkers

Abstract

Preterm birth (PTB) is the leading cause of neurological disabilities and infant mortality. Over one million infants died in 2013 from PTB related complications. Esplin, et al.1 found that PTB risk can be determined with 87% selectivity and 81% specificity through nine maternal blood serum biomarkers. One of these biomarkers is the thrombin-antithrombin III complex (TAT). TAT cannot be purchased so it must be synthesized in the lab to be analyzed. We have formed TAT in buffer and analyzed the results by dot blot test, capillary electrophoresis, and microchip electrophoresis. Once synthesized, we were able to separate TAT from six other PTB biomarkers using microchip electrophoresis. We are presently working to integrate this separation with upstream microfluidic sample preparation steps for the diagnosis of PTB risk.

Reference

1. Esplin, M.S.; Merrell, K.; Goldenberg, R.; et al. Am. J. Obstet. Gynecol. 2011, 204, 391.e1-8.