BYU

Abstract by Jacob Vance

Personal Infomation


Presenter's Name

Jacob Vance

Co-Presenters

Emily Tague

Degree Level

Undergraduate

Co-Authors

Matthew Hyer
Emily Tague

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Kenneth Christensen

Title

Obtaining multiplexed measurements in Trypanosoma brucei with the use of fluorescent staining

Abstract

Trypanosomes are a group of single-celled parasites, some of which are a major cause of neglected tropical diseases (NTDs) in humans. With the objective of identifying potential targets for drug action, our group has used Trypanosoma brucei as a model of parasite metabolism.  We have adapted genetically encoded fluorescence resonance energy transfer (FRET) sensor proteins to monitor levels of analytes that are relevant to glycolysis  in T. brucei. We have been successful in monitoring levels of a single analyte in response to changing conditions in the cell’s environment. However, glycolysis in the parasite is a complex pathway. By staining sensor-expressing cells with multiple fluorescent dyes, we have been able to obtain multiplexed measurements of multiple analytes in a single experiment. We demonstrate that this method can be used to obtain data for four different analytes in the same population.