BYU

Abstract by Nathan Zuniga

Personal Infomation


Presenter's Name

Nathan Zuniga

Co-Presenters

None

Degree Level

Undergraduate

Co-Authors

Richard Watt

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Richard Watt

Title

Glutathione and Homocystiene Interactions with Iron. Loading into Ferritin

Abstract

The cellular Labile Iron Pool (LIP) is a reservoir of iron for cell use. In vitro studies show Fe(II) is the physiological oxidation state for delivery to enzymes.  However, Fe (II) produces oxygen radicals (ROS). Hider proposed cells protect against ROS formation by complexing Fe(II) to Glutathione (GSH).  We report that the GS:Fe(II) complex is not oxidized by O2 confirming Hider’s hypothesis. Oxidative stress diseases have elevated ROS production and elevated Homocysteine (Hcy) levels.  We proposed that Hcy competes with GSH for Fe(II) and oxidizes Fe(II) to Fe(III) producing ROS.  Iron loading into ferritin was monitored with Fe(II), GS:Fe(II) and Hcy:Fe(II). Kinetics demonstrate GS:Fe(II) loads Ferritin but Hcy:Fe(II) reacts with ferritin to produce a purple color (580 nM). Our results demonstrate the interactions biological molecules with Fe homeostasis.