Abstract by Yonghoo Jo
Chemistry and Biochemistry
Stepped concentration barcoding for analysis of multiple biosensor readout to monitor glycolysis in African trypanosomes
Trypanosoma brucei (T. brucei) is a unicellular eukaryotic parasite that is transmitted by tsetse fly bites and causes a fatal neurodegenerative disease known as Human African Trypanosomiasis (HAT) with limited treatment options. Since glycolysis is the sole energy source of the bloodstream parasite life stage (BSF), our lab is identifying small molecules that specifically block glycolysis in the parasite using genetically encoded biosensors that provide a readout of glucose, ATP, and pH in live parasites via flow cytometry. While single analyte measurements are informative, multiplexing these and other analytes will provide a better understanding of T. brucei physiology and the mechanisms of action known compounds use to kill the parasite. To do this, we are barcoding cells expressing different biosensors with stepped concentrations of a fluorescent reporter and have optimized the conditions needed for simultaneous measurements of multiple analytes.