BYU

Abstract by Ellen Parker

Personal Infomation


Presenter's Name

Ellen Parker

Co-Presenters

None

Degree Level

Masters

Co-Authors

Mukul Sonker
Haifa Almughamsi
Hua Gong
Michael Beauchamp
Gregory Nordin
Adam Woolley

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Adam Woolley

Title

Affinity Extraction of Preterm Birth Biomarkers in 3D Printed Microfluidic Devices

Abstract

Preterm birth (PTB) affects 1 in 9 children born in the United States.  Our research focuses on developing 3D printed microfluidic devices for the quick and inexpensive analysis of a panel of PTB biomarkers found in maternal blood serum.  We have 3D printed a 45 μm by 40 μm microfluidic channel with a unique monolith exposure window.  In addition, conditions for the polymerization of affinity monoliths in these 3D printed devices have been optimized. A labeled PTB biomarker, ferritin, is electrophoresed through the monolith and detected by laser induced fluorescence.  The ability of the affinity monolith to retain and elute labeled ferritin is evaluated in order to determine the best conditions for extracting ferritin.  Demonstration of the extraction of ferritin is a key step in the development of an integrated 3D printed microfluidic device that can be used to predict PTB risk.