Abstract by Ellen Parker
Chemistry and Biochemistry
Affinity Extraction of Preterm Birth Biomarkers in 3D Printed Microfluidic Devices
Preterm birth (PTB) affects 1 in 9 children born in the United States. Our research focuses on developing 3D printed microfluidic devices for the quick and inexpensive analysis of a panel of PTB biomarkers found in maternal blood serum. We have 3D printed a 45 μm by 40 μm microfluidic channel with a unique monolith exposure window. In addition, conditions for the polymerization of affinity monoliths in these 3D printed devices have been optimized. A labeled PTB biomarker, ferritin, is electrophoresed through the monolith and detected by laser induced fluorescence. The ability of the affinity monolith to retain and elute labeled ferritin is evaluated in order to determine the best conditions for extracting ferritin. Demonstration of the extraction of ferritin is a key step in the development of an integrated 3D printed microfluidic device that can be used to predict PTB risk.