Abstract by Naomi Flindt
Chemistry and Biochemistry
Quantification of Iron in Biological Samples using XRF
Biological elemental analysis is a challenging task. Inductively coupled mass spectrometry (ICP-MS) is a popular method but has limitations due to laborious sample preparation, cost of equipment, and is destructive to samples. Quantitation in MS can be limited by ionization efficiency and complex sample matrices. In this study we evaluate portable x-ray fluorescence spectroscopy (XRF) as a cutting-edge technology for biological elemental analysis. We developed an array of methods for the elemental analysis of ferritin, liver cells, and liver tissue samples using Bruker 5i Tracer. Our methods using XRF are faster, easier, non-destructive and use fewer consumables compared with ICP-MS. This study demonstrates that laboratory portable XRF has the needed precision to be used in biological scientific applications to make advances related to metal ion homeostasis. These advances can be used to further the understand the association of Fe with inflammation in diseases like Alzheimer’s, Diabetes, and Cancer.