BYU

Abstract by Kyle Cutler

Personal Infomation


Presenter's Name

Kyle Cutler

Degree Level

Undergraduate

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

John Price

Title

Deuterater: An Analyte-Agnostic Refactoring of Kinetic Analysis Software for Deuterium-Labeled Metabolomics

Abstract

Deuterium labeling is a simple, economically feasible technique for monitoring in vivo turnover rates through metabolic isotope labeling. The prevalence of H2O in biological processes allows deuterons from D2O to be easily incorporated in vivo into newly synthesized molecules. This labeling method and mass spectrometry are both naturally analyte-agnostic, and that property should extend to associated analytical tools.

The measurement of individual protein turnover rates in vivo is a critical step in understanding how proteostasis is regulated by synthesis and degradation. Applying the same computational technique to lipids allows us to reliably calculate specific lipids’ turnover rates despite the additional steps required to calculate its deuterium enrichment.

This study seeks to expand on the Deuterater proteomics tool. We will present our work on making the core of the Deuterater program analyte-agnostic, and the steps taken to reach that goal.