BYU

Abstract by Kristina Kohler

Personal Infomation


Presenter's Name

Kristina Kohler

Degree Level

Undergraduate

Co-Authors

Chrissy Egbert
Tsz Yin Chan
Logan Larsen
Will Tenney

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Joshua Andersen

Title

TNK1 reveals a novel autoinhibitory function of the UBA domain

Abstract

TNK1 is a little-studied cytosolic kinase that promotes cancer proliferation and growth. Despite its oncogenic role in various cancer types, the mechanisms which regulate TNK1 are poorly understood.  Our previous research has shown that a C-terminus truncation mutant of TNK1 has upregulated activity.  We propose that the C-terminus of TNK1 contains a unique ubiquitin-association (UBA) domain that functions as an auto-inhibitory domain. Using predictive protein modeling and western blot analyses, we found that ubiquitin-TNK1 binding disrupts the kinase activity of TNK1. We further discovered that the UBA hinders TNK1 self-oligomerization. Together, our data suggest a novel mechanism of UBA-mediated kinase regulation in cancer.