BYU

Abstract by Christena Bentley

Personal Infomation


Presenter's Name

Christena Bentley

Co-Presenters

Kennedy Wandelt

Degree Level

Undergraduate

Co-Authors

Elisabeth Gates Nick Morrill
Amanda Howard Wessley Call
Fischer Summers Ally Okolowitz

Abstract Infomation


Department

Physics and Astronomy

Faculty Advisor

Robert Davis

Title

Effect of Hematocrit on Rapid Bacterial Extraction

Abstract

Current techniques for characterizing septic bacterial strains require a minimum of 24 hours for positive blood culture detection. The aim of this research is to develop a more efficient procedure for sample processing, which will improve upon current diagnosis and prescription practices. We are achieving rapid extraction of bacteria from blood by using magnetic nanoparticles (MNPs), modified to bind to bacteria. Preliminary data from our lab suggest that red blood cell (RBC) percentage, also known as hematocrit, interferes with our bacterial capture. To test the relationship between hematocrit and bacterial capture, we produced and processed blood samples diluted with plasma to effectively reduce the hematocrit. A negative relationship between bacterial capture and hematocrit was observed, suggesting that RBCs affect the binding of MNPs to bacteria. Data has provided us with a model by which we can account for these matrix effects in future samples.