Abstract by David Tripp

Personal Infomation

Presenter's Name

David Tripp

Degree Level



Jeremy Tsang
Kenneth Christensen
Kade Wheelwright
Cody Roberts

Abstract Infomation


Chemistry and Biochemistry

Faculty Advisor

Kenneth Christensen


Required Post-Translational Modifications of Capillary Morphogenesis Gene 2 for Co-Localization of ß-arrestin Upon Treatment of Anthrax Toxin


Capillary Morphogenesis Gene 2 (CMG2) is commonly known for its activity as an anthrax toxin receptor, internalizing CMG2 via endocytosis following binding of the anthrax protective antigen (PA) moiety of the binary toxin. ß-arrestin has been reported as being necessary for PA endocytosis, but the role it plays therein has yet to be completely understood. To investigate the role of CMG2 post-translational modifications on ß-arrestin recruitment after PA binding, our lab has generated fluorescent protein fusions of CMG2 mutants at the post-translational modification sites on its cytosolic tail. These mutants were individually transfected into HEK293T also expressing ß-arrestin-RFP. Since HEK293T cells do not natively express CMG2, we were able to systematically probe co-localization of CMG2 and ß-arrestin at the cell membrane and on endosomal membranes following PA treatment. We have identified important sites of post-translational modifications required to recruit ß-arrestin to CMG2 during endocytosis to begin to understand signaling pathways whereby CMG2 function.