BYU

Abstract by Taylor Lundgren

Personal Infomation


Presenter's Name

Taylor Lundgren

Degree Level

Undergraduate

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Steven Graves

Title

Developing Serum Diagnostic for Endometriosis by a Peptidomic Approach

Abstract

Endometriosis is a common, painful disease where the uterine lining grows elsewhere.  It affects ~10% of all women of reproductive age. It often results in infertility and increases risk of other diseases (1-3). Diagnosis typically takes years and requires laparoscopy and biopsy (4).  MRI or ultrasound are ineffective.  Having a blood test that could provide a diagnosis would be valuable.  A serum peptidomic approach was used to identify biomarkers with diagnostic potential. Serum came from endometriosis positive and negative patients: note controls were women having comparable symptoms but were surgically negative. Serum protein depletion was carried out by acetonitrile precipitation followed by reverse phase LC coupled to ESI-Q-TOF-MS. This found 32 molecules having p-values<0.05, resulting in an optimized set of 8 biomarkers with an internally verified AUC of 0.803. These biomarkers were characterized by tandem MS.  The results suggest a set of diagnostic markers can be found that would assist in endometriosis diagnosis.