Abstract by Taylor Lundgren
Chemistry and Biochemistry
Developing Serum Diagnostic for Endometriosis by a Peptidomic Approach
Endometriosis is a common, painful disease where the uterine lining grows elsewhere. It affects ~10% of all women of reproductive age. It often results in infertility and increases risk of other diseases (1-3). Diagnosis typically takes years and requires laparoscopy and biopsy (4). MRI or ultrasound are ineffective. Having a blood test that could provide a diagnosis would be valuable. A serum peptidomic approach was used to identify biomarkers with diagnostic potential. Serum came from endometriosis positive and negative patients: note controls were women having comparable symptoms but were surgically negative. Serum protein depletion was carried out by acetonitrile precipitation followed by reverse phase LC coupled to ESI-Q-TOF-MS. This found 32 molecules having p-values<0.05, resulting in an optimized set of 8 biomarkers with an internally verified AUC of 0.803. These biomarkers were characterized by tandem MS. The results suggest a set of diagnostic markers can be found that would assist in endometriosis diagnosis.