BYU

Abstract by Chrissy Egbert

Personal Infomation


Presenter's Name

Chrissy Egbert

Degree Level

Doctorate

Co-Authors

Tsz Yin Chan
Logan Larsen
Will Tenney
Kristina Kohler

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Joshua Andersen

Title

A ubiquitin mediated mechanism of TNK1 activity and stability

Abstract

TNK1 is a poorly characterized non-receptor protein tyrosine kinase (NRTK) with potential oncogenic function. We do not yet understand the mechanisms that regulate TNK1 signaling, activity and stability. TNK1 possesses a unique protein domain organization. Specifically, it contains a C-terminal ubiquitin association  (UBA) domain that is uncommon in NRTKs. We discovered that this UBA domain helps regulate TNK1 activity and stability. The removal of the TNK1 UBA domain leads to an increase in TNK1 kinase activity and overall TNK1 protein levels. Using predictive protein modeling we identified several important residues for ubiquitin association. Mutation of these residues leads to the loss of ubiquitin binding and a corresponding increase in TNK1 kinase activity. Finally, we identified TNK1 mutations in primary human cancers that disrupt the TNK1 UBA domain, suggesting that loss of TNK1 Ubiquitin binding may be a foundational oncogenic event in human cancer.