BYU

Abstract by Anna V Nielsen

Personal Infomation


Presenter's Name

Anna V Nielsen

Degree Level

Doctorate

Co-Authors

Benjamin Q George
Michael J Beauchamp

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Adam Woolley

Title

Solid Phase Extraction and Fluorescent Labeling of Preterm Birth Biomarkers in 3D Printed Microfluidic Devices

Abstract

Even though preterm birth (PTB) is the leading cause of infant mortality and neurological disabilities, no early clinical assessment currently exists to diagnose PTB risk prior to the onset of early labor. As a step to developing such a diagnostic tool, we are developing 3D printed microfluidic devices (50 x 50 µm channels) with reversed-phase monoliths for the capture, on-chip fluorescent labeling, and selective elution of non-specific adsorbants, excess fluorescent dye, then PTB biomarkers via the increase of acetonitrile concentration. We further demonstrate the potential to automate this analysis by integrating pneumatic pumps and valves into the devices. While this process has been performed previously on conventionally fabricated devices, we highlight the advantages of 3D printing for higher and faster device fabrication yields than traditional planar 2D fabrication methods as well as the potential for 3D printing to be applied to other clinical assessments.