Abstract by Jordan Coburn
Chemistry and Biochemistry
Antibiofilm activity of CSA-131 in poloxamer micelles against pathogenic bacteria and fungi
Ceragenins are cationic, bile acid-based antimicrobials developed to mimic the activities of endogenous antimicrobial peptides. The treatment of lung infections caused by cystic fibrosis has been identified as a potential use of ceragenins. However, we have observed that at high concentrations, ceragenins may damage cilia in the trachea. It is hypothesized that formulating ceragenins in poloxamer micelles will reduce damage to cilia while maintaining antimicrobial activity. This work quantifies the efficacy of a lead ceragenin, CSA-131, with and without pluronic micelles against bacteria and fungi. Treatment of established biofilms by CSA-131, both with and without pluronic showed a significant log reduction in tested bacteria and fungi. XTT colorimetric assays showed similar results. There was no significant hindrance in the total efficacy of CSA-131 due to the addition of pluronic. A study was also performed with CSA-131, with and without 4% pluronic, in which the two formulations had comparable kinetic antimicrobial activities.